Device

Part:BBa_K598019:Design

Designed by: Zhenrun ZHANG   Group: iGEM11_Peking_R   (2011-09-27)


B0015+pBAD+vioABD+B0015+pBAD+TPP Down-regulated Hammerhead Ribozyme 2.5+vioE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 262
    Illegal NheI site found at 1296
    Illegal NheI site found at 5973
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 202
    Illegal BamHI site found at 5913
    Illegal BamHI site found at 6105
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4081
    Illegal AgeI site found at 4277
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 5506
    Illegal SapI.rc site found at 5581


Design Notes

the Ribosomal Binding Site of vioE is contained in TPP Ribozyme.


Source

Hammerhead Ribozyme backbone comes from E.coli, and TPP aptamer comes from B.subtilis.

References